AbstractThe Southeastern Center for Microscopy of MacroMolecular Machines (SECM4) is a consortiumof 15 Universities/Medical Centers with a total of 19 investigators throughout the Eastern UnitedStates studying a wide range of important biomedical projects as variable as high resolutionvirus structure, membrane protein structure, macromolecular complexes of various types, someisolated in active form from cells, bacterial ultrastructure, muscle filaments, spliceosomes,ribosomes complexes all of which will benefit from ready access to a high resolution electronmicroscope such as a Titan Krios equipped with a direct electron detector (DED). Human healthimplications extend from virus and bacterial pathogens to the understanding of diseasesresulting form genetic mutations. The basic biology of cancer and heart disease is being studiedin several member laboratories. The Titan Krios at Florida State University has been inoperation since 2009 and recently has had its image recording device upgraded from CCDcamera to a Direct Electron LLC, DE-20 direct electron detector positioned ahead of an existingimaging filter which removes inelastically scattered electrons thereby improving the imagequality. Although we propose a robust plan to enable members to come to Florida StateUniversity, we propose creating a facility based on the synchrotron template currently in use atmultiple sites X-ray crystallography beam lines around the country whereby users shipspecimens to us and watch the data being collected as it comes off the microscope from thefamiliar confines of their own laboratories. We will provide sufficient preprocessing thatconsortium members can evaluate the prospects for obtaining a final high resolution structurefrom damage and motion corrected ?movie? images of their samples. The result will be a modelfor high throughput structure determination utilizing high-end instrumentation that can reveal theinner workings of complex macromolecules and subcellular structures.
|Effective start/end date||7/18/16 → 6/30/21|
- National Institutes of Health: $922,279.00