C57BL/6J mouse apolipoprotein A2 gene is deterministic for apnea

Carl B. Gillombardo, Rebecca Darrah, Thomas E. Dick, Michael Moore, Nathan Kong, Michael J. Decker, Fang Han, Motoo Yamauchi, Mathias Dutschmann, Sausan Azzam, Kingman P. Strohl

Research output: Contribution to journalArticle

Abstract

Rationale Brainstem apolipoprotein AII (apoa2) mRNA expression correlates with apnea in breathing present in the adult C57Bl/6J (B6) sleep apnea model. Objectives To test the hypothesis that the B6 apoa2 gene contributes to the trait, we performed plethysmographic testing in apoa2 knock out (KO: −/-) mice, an in situ brainstem-spinal cord preparation comparing KO to WT (+/+) mice, and B6xDBA recombinant inbred strains (RISs). Measurements and main results Apoa2 WT do, but KO and heterozygote (+/−) mice do not exhibit apnea during post-hypoxic breathing, measured in vivo. In the in situ model, pauses and instability in fictive phrenic bursting are substantially reduced in KO vs. WT preparations. In 24 RISs, apnea number in vivo was higher in strains with B6 apoa2 than with DBA apoa2 alleles. Conclusions The B6 apoa2 polymorphism is directly involved in breath production, and its identification suggests a novel pathway influencing risk for adult sleep apnea

Original languageEnglish (US)
Pages (from-to)88-94
Number of pages7
JournalRespiratory Physiology and Neurobiology
Volume235
DOIs
StatePublished - Jan 1 2017

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Apolipoprotein A-II
Apnea
Inbred C57BL Mouse
Genes
Sleep Apnea Syndromes
Brain Stem
Respiration
Heterozygote
Diaphragm
Knockout Mice
Spinal Cord
Alleles
Messenger RNA

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology
  • Pulmonary and Respiratory Medicine

Cite this

C57BL/6J mouse apolipoprotein A2 gene is deterministic for apnea. / Gillombardo, Carl B.; Darrah, Rebecca; Dick, Thomas E.; Moore, Michael; Kong, Nathan; Decker, Michael J.; Han, Fang; Yamauchi, Motoo; Dutschmann, Mathias; Azzam, Sausan; Strohl, Kingman P.

In: Respiratory Physiology and Neurobiology, Vol. 235, 01.01.2017, p. 88-94.

Research output: Contribution to journalArticle

Gillombardo, CB, Darrah, R, Dick, TE, Moore, M, Kong, N, Decker, MJ, Han, F, Yamauchi, M, Dutschmann, M, Azzam, S & Strohl, KP 2017, 'C57BL/6J mouse apolipoprotein A2 gene is deterministic for apnea' Respiratory Physiology and Neurobiology, vol 235, pp. 88-94. DOI: 10.1016/j.resp.2016.10.006

Gillombardo, Carl B.; Darrah, Rebecca; Dick, Thomas E.; Moore, Michael; Kong, Nathan; Decker, Michael J.; Han, Fang; Yamauchi, Motoo; Dutschmann, Mathias; Azzam, Sausan; Strohl, Kingman P. / C57BL/6J mouse apolipoprotein A2 gene is deterministic for apnea.

In: Respiratory Physiology and Neurobiology, Vol. 235, 01.01.2017, p. 88-94.

Research output: Contribution to journalArticle

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