Immuno-TEM/STEM in Retinal Research

Sanae Sakami, Krzysztof Palczewski

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Immuno-EM is a method that can determine the localization of a protein in a tissue at the ultrastructural level. Generally, membrane structures in immuno-EM specimens have very low contrast because fixation is performed without osmium. Here, by using high-angle annular dark field (HAADF)-scanning transmission electron microscopy (STEM) instead of transmission electron microscopy (TEM) for observation of immuno-EM samples, we demonstrate that photoreceptor disk membranes are clearly visible and that the procedures described in this article can be extended to visualize other membrane structures.

LanguageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages311-332
Number of pages22
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1834
ISSN (Print)1064-3745

Fingerprint

Scanning Transmission Electron Microscopy
Immunoelectron Microscopy
Transmission Electron Microscopy
Membranes
Research
Osmium
Observation
Proteins

Keywords

  • Disk (disc) membrane
  • HAADF-STEM
  • Immuno-EM
  • LR white resin and rhodopsin
  • Photoreceptor
  • TEM

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Sakami, S., & Palczewski, K. (2019). Immuno-TEM/STEM in Retinal Research. In Methods in Molecular Biology (pp. 311-332). (Methods in Molecular Biology; Vol. 1834). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8669-9_20

Immuno-TEM/STEM in Retinal Research. / Sakami, Sanae; Palczewski, Krzysztof.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 311-332 (Methods in Molecular Biology; Vol. 1834).

Research output: Chapter in Book/Report/Conference proceedingChapter

Sakami, S & Palczewski, K 2019, Immuno-TEM/STEM in Retinal Research. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1834, Humana Press Inc., pp. 311-332. https://doi.org/10.1007/978-1-4939-8669-9_20
Sakami S, Palczewski K. Immuno-TEM/STEM in Retinal Research. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 311-332. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8669-9_20
Sakami, Sanae ; Palczewski, Krzysztof. / Immuno-TEM/STEM in Retinal Research. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 311-332 (Methods in Molecular Biology).
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