Noninvasive Two-Photon Microscopy Imaging of Mouse Retina and Retinal Pigment Epithelium

Grazyna Palczewska, Tim S Kern, Krzysztof Palczewski

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Two-photon excitation microscopy is perfectly suited for imaging deep into the retina due to its use of infrared (IR) wavelengths to excite endogenous fluorophores such as vitamin A-derived retinoids present in this tissue. Furthermore, two-photon excitation occurs only around a small focal volume, and scattered IR photons cannot excite retinal chromophores. These characteristics contribute to subcellular resolution and low noise of images obtained from deep within retinal layers. Here we describe how to customize a two-photon microscope for noninvasive imaging of the retina and retinal pigment epithelium (RPE) in the mouse eye, along with detailed instructions for mouse handling and retinal imaging, and we provide examples of mouse retinal two-photon microscopy data.

LanguageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages333-343
Number of pages11
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1834
ISSN (Print)1064-3745

Fingerprint

Retinal Pigment Epithelium
Vitamin A
Photons
Retina
Microscopy
Retinoids
Noise

Keywords

  • Mouse eye
  • Retina
  • RPE
  • Two-photon microscopy
  • Vitamin A

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Palczewska, G., Kern, T. S., & Palczewski, K. (2019). Noninvasive Two-Photon Microscopy Imaging of Mouse Retina and Retinal Pigment Epithelium. In Methods in Molecular Biology (pp. 333-343). (Methods in Molecular Biology; Vol. 1834). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8669-9_21

Noninvasive Two-Photon Microscopy Imaging of Mouse Retina and Retinal Pigment Epithelium. / Palczewska, Grazyna; Kern, Tim S; Palczewski, Krzysztof.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 333-343 (Methods in Molecular Biology; Vol. 1834).

Research output: Chapter in Book/Report/Conference proceedingChapter

Palczewska, G, Kern, TS & Palczewski, K 2019, Noninvasive Two-Photon Microscopy Imaging of Mouse Retina and Retinal Pigment Epithelium. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1834, Humana Press Inc., pp. 333-343. https://doi.org/10.1007/978-1-4939-8669-9_21
Palczewska G, Kern TS, Palczewski K. Noninvasive Two-Photon Microscopy Imaging of Mouse Retina and Retinal Pigment Epithelium. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 333-343. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8669-9_21
Palczewska, Grazyna ; Kern, Tim S ; Palczewski, Krzysztof. / Noninvasive Two-Photon Microscopy Imaging of Mouse Retina and Retinal Pigment Epithelium. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 333-343 (Methods in Molecular Biology).
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