Single cell magnetometry by magnetophoresis vs. bulk cell suspension magnetometry by SQUID-MPMS – A comparison

Wei Xue, Lee R. Moore, Naruhiko Nakano, Jeffrey J. Chalmers, Maciej Zborowski

Research output: Contribution to journalArticle

Abstract

Paramagnetic constituents of a cell have strong effect on the cell's volume magnetic susceptibility even at low volume fraction because of their high susceptibility relative to that of the diamagnetic cell constituents. The effect can be measured at a single cell level by measuring cell terminal velocity in viscous media using a microscope equipped with a well-defined field and gradient magnet configuration (referred to as magnetophoretic analysis by cell tracking velocimetry, CTV). The sensitivity of such a microscopic-scale magnetometry was compared to that of a reference method of superconducting quantum interference-magnetic properties measurement system (SQUID-MPMS) using a red blood cell (RBC) suspension model. The RBC hemoglobin oxygen saturation determines the hemoglobin molecular magnetic susceptibility (diamagnetic when fully oxygenated, paramagnetic when fully deoxygenated or converted to methemoglobin). The SQUID-MPMS measurements were performed on an average of 5000 RBCs in 20 µL physiological phosphate buffer at room temperature, those by CTV on a single cell track in a mean magnetic field of 1.6 T and mean gradient of 240 T/m, repeated for an average of 1000 tracks per sample. This suggests 5000× higher sensitivity of cell susceptometry by magnetophoretic analysis than by SQUID-MPMS. The magnetophoretic mean RBC magnetic susceptibilities were in the range determined by SQUID-MPMS (lower limit) and theory (upper limit). The ability of magnetophoretic analysis to resolve susceptibility peaks in a mixed cell populations was confirmed for an oxy RBC and met RBC mixture. Magnetophoretic analysis by CTV provides new tool for studies of emergence of paramagnetic reaction products in the cell.

LanguageEnglish (US)
Pages152-160
Number of pages9
JournalJournal of Magnetism and Magnetic Materials
Volume474
DOIs
StatePublished - Mar 15 2019

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Hemoglobin oxygen saturation
shellfish
Molluscs
Shellfish
Paramagnetism
Vehicle suspensions
Molecular oxygen
paramagnetism
Hemoglobin
SQUIDs
Cell proliferation
hemoglobin
Magnetometers
Magnetic susceptibility
Cell culture
magnetometers
magnetic measurement
Suspensions
Hemoglobins
Blood

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Single cell magnetometry by magnetophoresis vs. bulk cell suspension magnetometry by SQUID-MPMS – A comparison. / Xue, Wei; Moore, Lee R.; Nakano, Naruhiko; Chalmers, Jeffrey J.; Zborowski, Maciej.

In: Journal of Magnetism and Magnetic Materials, Vol. 474, 15.03.2019, p. 152-160.

Research output: Contribution to journalArticle

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